Journal: Cell reports

Article Title: Unique molecular features and cellular responses differentiate two populations of motor cortical layer 5b neurons in a preclinical model of ALS

doi: 10.1016/j.celrep.2022.110556

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Donkey polyclonal anti-Chicken, Alexa 488-conjugated , Jackson Immunoresearch , Cat# 703-545-155; RRID: AB_2340375.

Techniques: Recombinant, RNAscope, Protease Inhibitor, Plasmid Preparation, Multiplex Assay, Positive Control, Software

Journal: Cell reports

Article Title: Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis

doi: 10.1016/j.celrep.2022.111742

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse IgG, HRP-linked Antibody , Cell Signaling Technology , Cat# 7076, RRID:AB_330924.

Techniques: Recombinant, Membrane, Wound Healing Assay, Invasion Assay, MTT Cell Proliferation, Protease Inhibitor, Western Blot, Lysis, In Situ Hybridization, Plasmid Preparation, cDNA Synthesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, RNAscope, RNA Sequencing, Gene Expression, Knock-Out, shRNA, CRISPR, Cloning, Software, Imaging

Journal: Cell reports

Article Title: Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis

doi: 10.1016/j.celrep.2022.111742

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Phospho-Akt (Ser473) Antibody , Cell Signaling Technology , Cat# 9271, RRID:AB_329825.

Techniques: Recombinant, Membrane, Wound Healing Assay, Invasion Assay, MTT Cell Proliferation, Protease Inhibitor, Western Blot, Lysis, In Situ Hybridization, Plasmid Preparation, cDNA Synthesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, RNAscope, RNA Sequencing, Gene Expression, Knock-Out, shRNA, CRISPR, Cloning, Software, Imaging

Journal: Cell Metabolism

Article Title: Nutrient-sensing AgRP neurons relay control of liver autophagy during energy deprivation

doi: 10.1016/j.cmet.2023.03.019

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal Phospho-AMPK (Thr172, 40H9) , Cell Signaling Technology , Cat# 2535S; RRID: AB_331250.

Techniques: Virus, Plasmid Preparation, Recombinant, Protease Inhibitor, Western Blot, Blocking Assay, In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Isolation, Bicinchoninic Acid Protein Assay, Reverse Transcription, RNAscope, Multiplex Assay, Phospho-proteomics, Software, Microscopy, Mass Spectrometry, Liquid Chromatography, Chromatography

Journal: Cell Metabolism

Article Title: Nutrient-sensing AgRP neurons relay control of liver autophagy during energy deprivation

doi: 10.1016/j.cmet.2023.03.019

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal Phospho-AKT (Ser473, D9E) , Cell Signaling Technology , Cat# 4060L; RRID: AB_2315049.

Techniques: Virus, Plasmid Preparation, Recombinant, Protease Inhibitor, Western Blot, Blocking Assay, In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Isolation, Bicinchoninic Acid Protein Assay, Reverse Transcription, RNAscope, Multiplex Assay, Phospho-proteomics, Software, Microscopy, Mass Spectrometry, Liquid Chromatography, Chromatography

Journal: Cell Metabolism

Article Title: Nutrient-sensing AgRP neurons relay control of liver autophagy during energy deprivation

doi: 10.1016/j.cmet.2023.03.019

Figure Lengend Snippet:

Article Snippet: Sections were then mounted with DAPI using Vectashield Antifade Mounting Medium (Vector Laboratories), covered using a coverslip and stored at 4°C in the dark.

Techniques: Virus, Plasmid Preparation, Recombinant, Protease Inhibitor, Western Blot, Blocking Assay, In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Isolation, Bicinchoninic Acid Protein Assay, Reverse Transcription, RNAscope, Multiplex Assay, Software, Microscopy, Mass Spectrometry, Liquid Chromatography, Chromatography

Journal: Neuron

Article Title: Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity

doi: 10.1016/j.neuron.2022.09.021

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: When indicated, neutralizing anti-Plexin-D1 antibody (5μg/ml) (R&D Systems Cat# AF4160) was added to the co-cultures at the time of HUVEC seeding.

Techniques: Recombinant, Protease Inhibitor, Transfection, Plasmid Preparation, Cloning, Mutagenesis, Reverse Transcription, Sample Prep, RNA Library Preparation, Knock-Out, Control, Quantitative RT-PCR, In Situ, Software, Expressing, CRISPR, RNAscope

Journal: Neuron

Article Title: Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity

doi: 10.1016/j.neuron.2022.09.021

Figure Lengend Snippet: (A) Immunohistochemistry for Plexin-D1 reveals restricted vascular expression at E11.5.

Article Snippet: When indicated, neutralizing anti-Plexin-D1 antibody (5μg/ml) (R&D Systems Cat# AF4160) was added to the co-cultures at the time of HUVEC seeding.

Techniques: Immunohistochemistry, Expressing

Journal: Neuron

Article Title: Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity

doi: 10.1016/j.neuron.2022.09.021

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: When indicated, neutralizing anti-Plexin-D1 antibody (5μg/ml) (R&D Systems Cat# AF4160) was added to the co-cultures at the time of HUVEC seeding.

Techniques: Recombinant, Protease Inhibitor, Transfection, Plasmid Preparation, Cloning, Mutagenesis, Reverse Transcription, Sample Prep, RNA Library Preparation, Knock-Out, Control, Quantitative RT-PCR, In Situ, Software, Expressing, CRISPR, RNAscope